LUPRIMA^TM

LUPRIMA^TM · CELL CULTURE QUALITY CONTROL

Mycoplasma detection,
Made simple.

PCR-based screening for Mycoplasma, Acholeplasma and Ureaplasma contamination in cell cultures and laboratory samples.

LUPRIMA^TM · 25 reactions · Lyophilized · Made in Chile

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For research use only (RUO).

Protect your cultures. Advance your research.

Routine screening.
Clear controls.

A conserved region of the 16S rRNA gene is the target of LUPRIMA’s primer set. An endpoint PCR workflow connects your culture supernatant to a gel-based readout, with internal and positive controls included.

1–5

Reported sensitivity

Limit of detection of 1–5 fg DNA per reaction.

Species coverage

Coverage of 24 species based on in silico analysis.

IC / +

Controls included

Internal control for PCR inhibition monitoring and a non-infectious positive DNA control.

PCR

Direct sample workflow

Heat-treated supernatant can be used directly, without a separate DNA extraction step.

Assay performance depends on sample preparation and reaction conditions.

Open LUPRIMA kit showing reagent tubes and packaging

LUPRIMA^TM · 25 reactions

Inside the kit

Supplied lyophilized. Reconstitute the Primers Mix and controls with nuclease-free water before use, following the volumes specified in the protocol. After reconstitution, keep the reagents refrigerated at 2–8 °C.

Primers MixSpecific primers targeting the conserved 16S rRNA gene region.
Internal Control (IC)DNA template to help identify inhibition of the PCR reaction.
Positive Control (C+)Non-infectious Mycoplasma DNA template for run validation.

Required from the user — not included in the kit: Taq polymerase, dNTPs, PCR buffer, MgCl₂ and nuclease-free water. The assay was configured with GoTaq® Master Mix (M7823, Promega).

From culture to readout

A straightforward PCR workflow

Prepare the supernatant, amplify the target and read the bands together with valid run controls.

Collect

100–200 µL of supernatant from a culture at 90–100% confluence.

Prepare

Heat at 95 °C for 5 minutes. Briefly centrifuge to pellet debris.

Amplify

Use 2 µL of prepared sample in a 25 µL PCR reaction. Run 40 cycles.

Separate

Load 10 µL onto a 1.5% agarose gel. Run at 100 V for about 45 minutes.

Interpret

Evaluate the ~270 bp target and ~190 bp internal control bands.

Thermocycler program & protocol diagram

Thermocycler program

Stage	Temperature	Time
Initial denaturation	95 °C	2 min
Denaturation · 40 cycles	95 °C	30 s
Annealing · 40 cycles	55 °C	1 min
Extension · 40 cycles	72 °C	30 s
Final extension	72 °C	5 min

Follow the complete product information for reaction composition, reagent volumes, controls and troubleshooting.

Protocol diagram from LUPRIMA product information: culture supernatant, heat treatment, centrifugation, PCR and agarose gel — Original protocol diagram from the supplied product information, Figure 1.

Gel-based interpretation

Read the bands.
Check the controls.

The protocol describes a target band at approximately 270 bp and an internal control band at approximately 190 bp.

Band pattern	Interpretation
~190 bp only	No Mycoplasma target detected; internal control amplified.
~270 + ~190 bp	Target detected, with internal control amplification.
Strong ~270 bp only	Positive pattern described for highly contaminated samples.

Absence of both bands must not be interpreted as a negative result. Band intensity is not a quantitative measurement of contamination.

Use valid positive and negative run controls. Refer to the full protocol for troubleshooting and interpretation.

LUPRIMA agarose gel showing positive and negative controls, DNA ladder, contaminated samples and a non-contaminated sample — Representative 1.5% agarose gel reproduced from the product information, Figure 2. DNA ladder: AccuRuler 100 bp Plus.

Meet LUPRIMA

Made for the lab

Explore the packaging, reagent format and kit presentation.

LUPRIMA kit and reagent tubes, angled packaging view

Open LUPRIMA kit with three reagent tubes

LUPRIMA Mycoplasma Detection Kit front packaging view

Technical information

Specifications at a glance

An endpoint PCR assay for research laboratories working with cell cultures and biological samples.

Species coverage

Coverage of the following 24 species is based on in silico analysis. See Table 2 in the product information for amplicon sizes and detailed analysis.

Mycoplasma faucium, M. hyorhinis, M. orale, M. arginini, M. fermentans, M. salivarium, M. pulmonis, M. bovis, M. falconis, M. arthritidi, M. spermatophilum, M. opalescens, M. primatum, M. maculosa, M. cloacale, M. hyosynoviae, M. synoviae, M. penetrans, M. hominis, M. pirum, M. genitalium, M. pneumoniae, Acholeplasma laidlawii and Ureaplasma urealyticum.

Product	LUPRIMA^TM
Format	25 reactions · lyophilized components · reconstitution required
Method	Endpoint PCR · 16S rRNA gene
Reported LoD	1–5 fg DNA/reaction
Readout	1.5% agarose gel electrophoresis
Intended use	Research use only (RUO)

Storage & handling

Lyophilized

Shipping & receipt

The kit may be received at room temperature. Refrigerated storage at 2–8 °C is recommended upon receipt.

2–8 °C

After reconstitution

Keep reconstituted primers and controls refrigerated at 2–8 °C.

−20 °C

Long-term aliquots

Aliquot and freeze for long-term storage. Avoid repeated freeze–thaw cycles.

Storage information on this page reflects the updated handling guidance provided by Luyef. The downloadable protocol contains an earlier post-rehydration storage instruction.

Luyef Biotechnologies

Bring LUPRIMA
to your laboratory.

Contact our team for availability, quotations and technical support.

Contact our team ↗

solutions@luyef.com

Product documents

Product information & full protocol
Spanish / English · PDF ↗Product brochure
Spanish · PDF ↗

For research use only. Not intended for diagnostic procedures. Designed for detection of Mycoplasma contamination in cell cultures and laboratory samples. This assay has not been validated for clinical or diagnostic use in humans or animals.

LUPRIMA^TM

Mycoplasma detection,
Made simple.