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LUPRIMATM
LUPRIMATM · CELL CULTURE QUALITY CONTROL
Mycoplasma detection,
Made simple.
PCR-based screening for Mycoplasma, Acholeplasma and Ureaplasma contamination in cell cultures and laboratory samples.
LUPRIMATM · 25 reactions · Lyophilized · Made in Chile
For research use only (RUO).
Protect your cultures. Advance your research.
Routine screening. Clear controls.
A conserved region of the 16S rRNA gene is the target of LUPRIMA’s primer set. An endpoint PCR workflow connects your culture supernatant to a gel-based readout, with internal and positive controls included.
24
Species coverage
Coverage of 24 species based on in silico analysis.IC / +
Controls included
Internal control for PCR inhibition monitoring and a non-infectious positive DNA control.PCR
Direct sample workflow
Heat-treated supernatant can be used directly, without a separate DNA extraction step.Assay performance depends on sample preparation and reaction conditions.
LUPRIMATM · 25 reactions
Inside the kit
Supplied lyophilized. Reconstitute the Primers Mix and controls with nuclease-free water before use, following the volumes specified in the protocol. After reconstitution, keep the reagents refrigerated at 2–8 °C.
- Primers MixSpecific primers targeting the conserved 16S rRNA gene region.
- Internal Control (IC)DNA template to help identify inhibition of the PCR reaction.
- Positive Control (C+)Non-infectious Mycoplasma DNA template for run validation.
Required from the user — not included in the kit: Taq polymerase, dNTPs, PCR buffer, MgCl₂ and nuclease-free water. The assay was configured with GoTaq® Master Mix (M7823, Promega).
From culture to readout
A straightforward PCR workflow
Prepare the supernatant, amplify the target and read the bands together with valid run controls.
Collect
100–200 µL of supernatant from a culture at 90–100% confluence.Prepare
Heat at 95 °C for 5 minutes. Briefly centrifuge to pellet debris.Amplify
Use 2 µL of prepared sample in a 25 µL PCR reaction. Run 40 cycles.Separate
Load 10 µL onto a 1.5% agarose gel. Run at 100 V for about 45 minutes.Interpret
Evaluate the ~270 bp target and ~190 bp internal control bands.Thermocycler program & protocol diagram
Thermocycler program
| Stage | Temperature | Time |
|---|---|---|
| Initial denaturation | 95 °C | 2 min |
| Denaturation · 40 cycles | 95 °C | 30 s |
| Annealing · 40 cycles | 55 °C | 1 min |
| Extension · 40 cycles | 72 °C | 30 s |
| Final extension | 72 °C | 5 min |
Follow the complete product information for reaction composition, reagent volumes, controls and troubleshooting.
Gel-based interpretation
Read the bands. Check the controls.
The protocol describes a target band at approximately 270 bp and an internal control band at approximately 190 bp.
| Band pattern | Interpretation |
|---|---|
| ~190 bp only | No Mycoplasma target detected; internal control amplified. |
| ~270 + ~190 bp | Target detected, with internal control amplification. |
| Strong ~270 bp only | Positive pattern described for highly contaminated samples. |
Absence of both bands must not be interpreted as a negative result. Band intensity is not a quantitative measurement of contamination.
Use valid positive and negative run controls. Refer to the full protocol for troubleshooting and interpretation.
Meet LUPRIMA
Made for the lab
Explore the packaging, reagent format and kit presentation.
Technical information
Specifications at a glance
An endpoint PCR assay for research laboratories working with cell cultures and biological samples.
Species coverage
Coverage of the following 24 species is based on in silico analysis. See Table 2 in the product information for amplicon sizes and detailed analysis.
Mycoplasma faucium, M. hyorhinis, M. orale, M. arginini, M. fermentans, M. salivarium, M. pulmonis, M. bovis, M. falconis, M. arthritidi, M. spermatophilum, M. opalescens, M. primatum, M. maculosa, M. cloacale, M. hyosynoviae, M. synoviae, M. penetrans, M. hominis, M. pirum, M. genitalium, M. pneumoniae, Acholeplasma laidlawii and Ureaplasma urealyticum.
| Product | LUPRIMATM |
|---|---|
| Format | 25 reactions · lyophilized components · reconstitution required |
| Method | Endpoint PCR · 16S rRNA gene |
| Reported LoD | 1–5 fg DNA/reaction |
| Readout | 1.5% agarose gel electrophoresis |
| Intended use | Research use only (RUO) |
Storage & handling
Shipping & receipt
The kit may be received at room temperature. Refrigerated storage at 2–8 °C is recommended upon receipt.After reconstitution
Keep reconstituted primers and controls refrigerated at 2–8 °C.Long-term aliquots
Aliquot and freeze for long-term storage. Avoid repeated freeze–thaw cycles.Storage information on this page reflects the updated handling guidance provided by Luyef. The downloadable protocol contains an earlier post-rehydration storage instruction.
Luyef Biotechnologies
Bring LUPRIMA to your laboratory.
Contact our team for availability, quotations and technical support.LAUNCH PRICE
Valid until December 31, 2026
US$195 + VAT
25 reactions · ~US$7.80 per reaction
Regular price: US$300 + VAT
Developed and manufactured by Luyef Biotechnologies in Chile. Regional and global shipping available.
For research use only. Not intended for diagnostic procedures. Designed for detection of Mycoplasma contamination in cell cultures and laboratory samples. This assay has not been validated for clinical or diagnostic use in humans or animals.
LUPRIMA™ · Direct purchase
Bring LUPRIMA to your lab.
Made in Chile for faster, simpler access across LATAM. Request your kit by bank transfer. Our team will confirm availability, shipping and the final total before payment.
LUPRIMA™
Mycoplasma Detection Kit
25 reactions · Lyophilized
Launch price · per kit
US$199+ applicable taxes
Available through December 31, 2026.
Regular price: US$299 + taxes from January 1, 2027.
Payment options
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